When environmental conditions become unfavorable, tardigrades can enter a state of dormancy called “cryptobiosis,” which is characterized by desiccation, reduced metabolic rate, and enhanced resistance to conditions such as drought, low oxygen, salinity changes, or extreme temperatures. A dormant tardigrade, called a “tun,” may survive for long periods – 10 to 100 years, alternating between periods of activity and dormancy. (Nelson, 2002; Ruppert et al., 2004).
The Tardigrada are microscopic animals that look superficially like miniature eight-legged teddy bears. They live in habitats with at least intermittent moisture and can form resistant resting stages enabling them to endure extreme environmental conditions, including intense heat and cold, radiation, desiccation, even the vacuum of space. As of 2002, about 900 species had been described, but it’s likely that many more are still to be discovered.
(Becquerel, 1950; Crowe et al., 1998; Nelson, 2002; Ruppert et al., 2004; Horikawa et al., 2006; Jönsson et al., 2008)
The dormant state of cryptobiosis facilitates dispersal – the dried “tuns” are easily transported by winds or other animals (Ruppert et al., 2004).
Worldwide.
Tardigrades are found in a variety of wet or moist habitats, including marine and freshwater, moist soils, hot-springs, glaciers, mosses, lichens, leaf-litter. In environments with intermittent moisture, tardigrades can enter a “cryptobiotic phase” that allows them to survive dry periods. (Nelson 2002; Ruppert et al., 2004)
10 to 100 years (Ruppert et al., 2004).
Tardigrades are bilaterally symmetrical, usually “plump” and roughly cylindrical. Superficial form appears to be organized into five segments: a short head and a trunk of four segments, though the trunk may actually have 4 to 5 segments and the head 3 segments. Stubby legs are borne on each trunk segment, each having 4 to 8 retractile claws at the ends. The epidermis secretes a complex cuticle, which in some species is elaborated with structures like spines, granules, and pores. The cuticle is molted periodically. Tardigrades can be translucent or opaque, and the cuticle or gut may be colored brown, green, orange, pink, red, or yellow (Nelson, 2002; Ruppert et al., 2004)
Because tardigrades are so small, they have a high surface-area to volume ratio, making specialized gas exchange structures unnecessary. Diffusion across the body surface is sufficient. The nervous system is similar to that of arthropods, onychophorans, and annelids, with a lobed dorsal brain and ventral nerve cord with fused ganglia. (Nelson, 2002; Ruppert et al., 2004).
Most tardigrades are either female or male (“gonochoric”), though a few species are hermaphroditic. And males are absent in some parthenogenetic genera. (Nelson, 2002; Ruppert et al., 2004).
Body length is typically 100 to 150 micrometers, but there are that reach lengths of 1.5 mm (Nelson, 2002; Ruppert et al., 2004).
“Recent molecular analyses and additional morphological studies of the nervous system have confirmed the phylogenetic position of tardigrades as a sister group of the arthropods.” (Nelson, 2002)
However according to Ruppert et al. (2004), evolutionary relationships are currently uncertain. Though they seem to be closest to arthropods, there is also similarity to cycloneuralians (Kinorhynchans, Loriciferans, Priapulids, Nematodes, and Nematomorphs).
Trophic strategies vary. Most tardigrades feed on the cytoplasm of plant cells. Those living in soil eat algae, probably detritus, and some are predators, feeding on other microscopic soil animals, including nematodes and other tardigrades (Ruppert et al., 2004).
Tardigrades (/ˈtɑːrdɪˌɡreɪdz/),[1] known colloquially as water bears or moss piglets,[2][3][4][5] are a phylum of eight-legged segmented micro-animals.[2][6] They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ("little water bear").[7] In 1777, the Italian biologist Lazzaro Spallanzani named them Tardigrada (/tɑːrˈdɪɡrədə/), which means "slow steppers".[8]
Popular culture has recently elevated awareness of tardigrades in fictional settings and with fictional attributes, likely extending on the fact of their (actual) extreme hardiness. However, this evidences a perception "assignment problem" with tardigrades: as they branched off of the evolutionary tree extremely long ago, it is difficult to place them in a conventional context, e.g. the "arthropods" spiders, crabs, etc., to which they are likely distantly related, but do not belong.
They have been found in diverse regions of Earth's biosphere – mountaintops, the deep sea, tropical rainforests, and the Antarctic.[8] Tardigrades are among the most resilient animals known,[9][10] with individual species able to survive extreme conditions – such as exposure to extreme temperatures, extreme pressures (both high and low), air deprivation, radiation, dehydration, and starvation – that would quickly kill most other known forms of life.[11] Tardigrades have survived exposure to outer space.[12][13] There are about 1,300 known species[14] in the phylum Tardigrada, a part of the superphylum Ecdysozoa consisting of animals that grow by ecdysis such as arthropods and nematodes. The earliest known true members of the group are known from Cretaceous (145 to 66 million years ago) amber, found in North America, but are essentially modern forms, and therefore likely have a significantly earlier origin, as they diverged from their closest relatives in the Cambrian, over 500 million years ago.
Tardigrades are usually about 0.5 mm (0.020 in) long when fully grown.[2] They are short and plump, with four pairs of legs, each ending in claws (usually four to eight) or suction disks.[2][15] Tardigrades are prevalent in mosses and lichens and feed on plant cells, algae, and small invertebrates. When collected, they may be viewed under a low-power microscope, making them accessible to students and amateur scientists.[16]
Johann August Ephraim Goeze originally named the tardigrade Kleiner Wasserbär, meaning "little water-bear" in German (today, they are often referred to in German as Bärtierchen or "little bear-animal"). The name "water-bear" comes from the way they walk, reminiscent of a bear's gait. The name Tardigradum means "slow walker" and was given by Lazzaro Spallanzani in 1777.[8]
The largest adults may reach a body length of 1.5 mm (0.059 in), the smallest below 0.1 mm (0.0039 in). Newly hatched tardigrades may be smaller than 0.05 mm (0.0020 in). For comparison, grass pollen is typically 0.025–0.04 mm (0.00098–0.00157 in).
Tardigrades are often found on lichens and mosses, for example by soaking a piece of moss in water.[17] Other environments in which they are found include dunes and coasts generally, soil, leaf litter, and marine or freshwater sediments, where they may occur quite frequently, up to 25,000 animals per litre (95,000 animals per gallon). One tardigrade, Echiniscoides wyethi,[18] may be found on barnacles.[19]
Tardigrades have barrel-shaped bodies with four pairs of stubby legs. Most range from 0.3 to 0.5 mm (0.012 to 0.020 in) in length, although the largest species may reach 1.2 mm (0.047 in).[8] The body consists of a head, three body segments each with a pair of legs, and a caudal segment with a fourth pair of legs. The legs are without joints, while the feet have four to eight claws each. The cuticle contains chitin and protein and is moulted periodically. The first three pairs of legs are directed downward along the sides and are the primary means of locomotion, while the fourth pair is directed backward on the last segment of the trunk and is used primarily for grasping the substrate.[20]
Tardigrades lack several Hox genes and a large intermediate region of the body axis. In insects, this corresponds to the entire thorax and the abdomen. Practically the whole body, except for the last pair of legs, is made up of just the segments that are homologous to the head region in arthropods.[21]
All adult tardigrades of the same species have the same number of cells (see eutely). Some species have as many as 40,000 cells in each adult, while others have far fewer.[22][23]
The body cavity consists of a haemocoel, but the only place where a true coelom can be found is around the gonad. No respiratory organs are found, with gas exchange able to occur across the entirety of the body. Some tardigrades have three tubular glands associated with the rectum; these may be excretory organs similar to the Malpighian tubules of arthropods, although the details remain unclear.[24] Also, nephridia are absent.[25]
The tubular mouth is armed with stylets, which are used to pierce the plant cells, algae, or small invertebrates on which the tardigrades feed, releasing the body fluids or cell contents. The mouth opens into a triradiate, muscular, sucking pharynx. The stylets are lost when the animal molts, and a new pair is secreted from a pair of glands that lie on either side of the mouth. The pharynx connects to a short esophagus, and then to an intestine that occupies much of the length of the body, which is the main site of digestion. The intestine opens, via a short rectum, to an anus located at the terminal end of the body. Some species only defecate when they molt, leaving the feces behind with the shed cuticle.[24]
The brain develops in a bilaterally symmetric pattern.[26] Tardigrades have a dorsal brain atop a paired ventral nervous system. The brain includes multiple lobes, mostly consisting of three bilaterally paired clusters of neurons.[27] The brain is attached to a large ganglion below the esophagus, from which a double ventral nerve cord runs the length of the body. The cord possesses one ganglion per segment, each of which produces lateral nerve fibres that run into the limbs. Many species possess a pair of rhabdomeric pigment-cup eyes, and numerous sensory bristles are on the head and body.[28]
Tardigrades all possess a buccopharyngeal apparatus (swallowing device made of muscles and spines that activates an inner jaw and begins digestion and movement along the throat and intestine[29]) which, along with the claws, is used to differentiate species.
Although some species are parthenogenic, both males and females are usually present, although females are frequently larger and more common. Both sexes have a single gonad located above the intestine. Two ducts run from the testes in males, opening through a single pore in front of the anus. In contrast, females have a single duct opening either just above the anus or directly into the rectum, which forms a cloaca.[24]
Tardigrades are oviparous, and fertilization is usually external. Mating occurs during the molt with the eggs being laid inside the shed cuticle of the female and then covered with sperm. A few species have internal fertilization, with mating occurring before the female fully sheds her cuticle. In most cases, the eggs are left inside the shed cuticle to develop, but some species attach them to nearby substrate.[24]
The eggs hatch after no more than 14 days, with the young already possessing their full complement of adult cells. Growth to the adult size occurs by enlargement of the individual cells (hypertrophy), rather than by cell division. Tardigrades may molt up to 12 times.[24]
Tardigrades tend to court before mating. Courtship is an early step in mating and was first observed in tardigrades in 1895. Research shows that up to nine males aggregate around a female to mate.[30]
Most tardigrades are phytophagous (plant eaters) or bacteriophagous (bacteria eaters), but some are carnivorous to the extent that they eat smaller species of tardigrades (e.g., Milnesium tardigradum).[31][32]
Tardigrades share morphological characteristics with many species that differ largely by class. Biologists have a difficult time finding verification among tardigrade species because of this relationship. These animals are most closely related to the early evolution of arthropods.[33] Tardigrade fossils go as far back as the Cretaceous period in North America. Tardigrades are considered cosmopolitan and can be located in regions all over the world. The eggs and cysts of tardigrades are so durable that they can be carried great distances on the feet of other animals.[15]
Tardigrades have survived all five recognized mass extinctions due to their plethora of survival characteristics, including the ability to survive conditions that would be fatal to almost all other animals (see the next section).
The lifespan of tardigrades ranges from three to four months for some species, up to two years for other species, not counting their time in dormant states.[34]
Scientists have reported tardigrades in hot springs, on top of the Himalayas[35] (6,000 m; 20,000 ft, above sea level) to the deep sea (−4,000 m; −13,000 ft) and from the polar regions to the equator, under layers of solid ice, and in ocean sediments. Many species can be found in milder environments such as lakes, ponds, and meadows, while others can be found in stone walls and roofs. Tardigrades are most common in moist environments, but can stay active wherever they can retain at least some moisture.
Tardigrades are thought to be able to survive even complete global mass extinction events caused by astrophysical events, such as gamma-ray bursts, or large meteorite impacts.[9][10] Some of them can withstand extremely cold temperatures down to 0.01 K (−460 °F; −273 °C) (close to absolute zero), while others can withstand extremely hot temperatures up to 420 K (300 °F; 150 °C)[36][37] for several minutes, pressures about six times greater than those found in the deepest ocean trenches, ionizing radiation at doses hundreds of times higher than the lethal dose for a human, and the vacuum of outer space.[38] Tardigrades that live in harsh conditions undergo an annual process of cyclomorphosis, allowing for survival in subzero temperatures.[39]
They are not considered extremophilic because they are not adapted to exploit these conditions, only to endure them. This means that their chances of dying increase the longer they are exposed to the extreme environments,[8] whereas true extremophiles thrive in a physically or geochemically extreme environment that would harm most other organisms.[3][40][41]
Tardigrades are one of the few groups of species that are capable of suspending their metabolism (see cryptobiosis). While in this state, their metabolism lowers to less than 0.01% of normal and their water content can drop to 1% of normal,[38] and they can go without food or water for more than 30 years, only to later rehydrate, forage, and reproduce.[3][42][43][44][45] Many species of tardigrade can survive in a dehydrated state up to five years, or longer in exceptional cases.[46][47] Depending on the environment, they may enter this state via anhydrobiosis, allowing tardigrades, along with some other micro-metazoans (such as worms, rotifers, and crustaceans), protozoans and plants, that ability to survive in inhospitable habitats, as opposed to other living things. In addition to offering protection from desiccation and freezing under normal circumstances, anhydrobiosis also permits resistance to unnatural abiotic extremes such as subzero temperatures,[48] cryobiosis, osmobiosis, or anoxybiosis.
Their ability to remain desiccated for such long periods of time was thought to be dependent on high levels of the nonreducing disaccharide trehalose,[49] which is commonly seen in other organisms that survive desiccation, and tardigrades have trehalase genes.[50] However, it has been seen that in both tardigrades and bdelloid rotifers, there is only a partial capability to synthesize trehalose in quantities that may contribute to desiccation tolerance.[49][51]
In response to this finding, more research was done on how these animals survived such extreme conditions. It was found that intrinsically disordered proteins (IDPs) were highly expressed in response to desiccation in tardigrades. Additionally, three new IDPs were found to be specific to tardigrades and coined tardigrade specific proteins (TDPs). These TDPs may maintain the structure of membranes by associating with the polar heads of the phospholipids bilayers, avoiding structural damage upon rehydration.[52] Also, TDPs, being highly hydrophilic, are thought to be involved in a vitrification mechanism, where a glass-like matrix forms within cells to protect the cellular contents upon desiccation.[53] Their DNA is further protected from radiation by a protein called "dsup" (short for damage suppressor).[54][55] In this cryptobiotic state, the tardigrade is known as a tun.[56]
Tardigrades are able to survive in extreme environments that would kill almost any other animal.[50] Extremes at which tardigrades can survive include those of:
Research published in 2020 shows that tardigrades are sensitive to high temperatures. Researchers showed it takes 48 hours at 37.1 °C (98.8 °F) to kill half of active tardigrades that have not been acclimated to heat. Acclimation boosted the temperature needed to kill half of active tardigrades to 37.6 °C (99.7 °F). Tardigrades in the tun state fared a bit better, tolerating higher temperatures. It took heating to 82.7 °C (180.9 °F) to kill half of tun-state tardigrades within one hour. Longer exposure time decreased the temperature needed for lethality, though. For 24 hours of exposure, 63.1 °C (145.6 °F) was enough to kill half of the tun-state tardigrades.[60]
Tardigrades are the first known animal to survive after exposure to outer space.[69] In September 2007, dehydrated tardigrades were taken into low Earth orbit on the FOTON-M3 mission carrying the BIOPAN astrobiology payload. For 10 days, groups of tardigrades, some of them previously dehydrated, some of them not, were exposed to the hard vacuum of outer space, or vacuum and solar UV radiation.[70][3][71][72] Back on Earth, more than 68% of the subjects protected from solar UV radiation were reanimated within 30 minutes following rehydration, although subsequent mortality was high; many of these produced viable embryos.[70][69] In contrast, hydrated samples exposed to the combined effect of vacuum and full solar UV radiation had significantly reduced survival, with only three subjects of Milnesium tardigradum surviving.[70] Also, it was found that the space vacuum did not have a significant effect on egg-laying in either R. coronifer or M. tardigradum. However, M. tardigradum exposed to UV radiation had a lower egg laying rate.[73] In May 2011, Italian scientists sent tardigrades on board the International Space Station along with extremophiles on STS-134, the final flight of Space Shuttle Endeavour.[74][75][76] Their conclusion was that microgravity and cosmic radiation "did not significantly affect survival of tardigrades in flight, and stated that tardigrades represent a useful animal for space research."[77][78] In November 2011, they were among the organisms to be sent by the U.S.-based Planetary Society on the Russian Fobos-Grunt mission's Living Interplanetary Flight Experiment to Phobos; however, the launch failed. In August 2019, scientists reported that a capsule containing tardigrades in a cryptobiotic state may have survived for a while on the Moon after the April 2019 crash landing of Beresheet, a failed Israeli lunar lander, but in May 2021 it was reported that they were unlikely to have survived the impact.[79][80][61]
In recent years, there has also been increased speculation regarding tardigrades' ability to survive on Mars without any life support systems,[81] but despite the hardiness of the tardigrade, it would still "need stuff to eat," in order to set up shop.[82]
Scientists have conducted morphological and molecular studies to understand how tardigrades relate to other lineages of ecdysozoan animals. Two plausible placements have been proposed: tardigrades are either most closely related to Arthropoda and Onychophora, or to nematodes. Evidence for the former is a common result of morphological studies; evidence of the latter is found in some molecular analyses.
The latter hypothesis has been rejected by recent microRNA and expressed sequence tag analyses.[83] Apparently, the grouping of tardigrades with nematodes found in a number of molecular studies is a long branch attraction artifact. Within the arthropod group (called panarthropoda and comprising onychophora, tardigrades and euarthropoda), three patterns of relationship are possible: tardigrades sister to onychophora plus arthropods (the lobopodia hypothesis); onychophora sister to tardigrades plus arthropods (the tactopoda hypothesis); and onychophora sister to tardigrades.[84] Recent analyses indicate that the panarthropoda group is monophyletic, and that tardigrades are a sister group of Antennopoda, the lineage consisting of arthropods and Onychophora.[83][85]
PanarthropodaWater bears (Tardigrada) .png)
Velvet worms (Onychophora) 
Arthropods (Arthropoda) 
The minute sizes of tardigrades and their membranous integuments make their fossilization both difficult to detect and highly unusual. The only known fossil specimens are those from mid-Cambrian deposits in Siberia (Orsten fauna) and a few rare specimens from Cretaceous amber.[86]
The Siberian tardigrade fossils differ from living tardigrades in several ways. They have three pairs of legs rather than four, they have a simplified head morphology, and they have no posterior head appendages, but they share with modern tardigrades their columnar cuticle construction.[87] Scientists think they represent a stem group of living tardigrades.[86]
In October 2021, a new species, Paradoryphoribius chronocaribbeus, was discovered as a fossil in amber that was dated to be 16 million years old.[88]
There are multiple lines of evidence that tardigrades are secondarily miniaturized from a larger ancestor,[89] probably a lobopodian and perhaps resembling Aysheaia, which many analyses place close to the divergence of the tardigrade lineage.[90][91] An alternative hypothesis derives tactopoda from a clade encompassing dinocaridids and Opabinia.[92]
The oldest remains of modern tardigrades are those of Milnesium swolenskyi, belonging to the living genus Milnesium known from a Late Cretaceous (Turonian) aged specimen of New Jersey amber, around 90 million years old. Another fossil, Beorn leggi, is known from a Late Campanian (~72 million years old) specimen of Canadian amber[93] and has been placed in its own family (Beornidae), but was subsequently suggested to belong to the Hypsibiidae. An indeterminate heterotardigrade was also noted from the same deposit.[94]
Tardigrade genomes vary in size, from about 75 to 800 megabase pairs of DNA.[95] Hypsibius exemplaris (formerly Hypsibius dujardini) has a compact genome of 100 megabase pairs[96] and a generation time of about two weeks; it can be cultured indefinitely and cryopreserved.[97]
The genome of Ramazzottius varieornatus, one of the most stress-tolerant species of tardigrades, was sequenced by a team of researchers from the University of Tokyo in 2015. While previous research had claimed that around one-sixth of the genome had been acquired from other organisms,[98] it is now known that less than 1.2% of its genes were the result of horizontal gene transfer. They also found evidence of a loss of gene pathways that are known to promote damage due to stress. This study also found a high expression of novel tardigrade-unique proteins, including Damage suppressor (Dsup), which was shown to protect against DNA damage from X-ray radiation. The same team applied the Dsup protein to human cultured cells and found that it suppressed X-ray damage to the human cells by around 40%.[55] While the exact mechanism of DNA protection is largely unknown, the results from an August 2020 study suggest that strong electrostatic attractions along with high protein flexibility help form a molecular aggregate, which allows Dsup to shield DNA.[99]
The Dsup proteins of tardigrades Ramazzottius varieornatus and Hypsibius exemplaris promote survival by binding to nucleosomes and protecting chromosomal DNA from hydroxyl radicals.[100] The Dsup protein of R. varieornatus also confers resistance to ultraviolet-C by upregulating DNA repair genes that protect the genomic DNA from the damages introduced by UV irradiation.[101]
Many organisms that live in aquatic environments feed on species such as nematodes, tardigrades, bacteria, algae, mites, and collembolans.[102] Tardigrades work as pioneer species by inhabiting new developing environments. This movement attracts other invertebrates to populate that space, while also attracting predators.[33]
{{cite journal}}: Cite journal requires |journal= (help) {{cite book}}: CS1 maint: others (link) Tardigrades (/ˈtɑːrdɪˌɡreɪdz/), known colloquially as water bears or moss piglets, are a phylum of eight-legged segmented micro-animals. They were first described by the German zoologist Johann August Ephraim Goeze in 1773, who called them Kleiner Wasserbär ("little water bear"). In 1777, the Italian biologist Lazzaro Spallanzani named them Tardigrada (/tɑːrˈdɪɡrədə/), which means "slow steppers".
Popular culture has recently elevated awareness of tardigrades in fictional settings and with fictional attributes, likely extending on the fact of their (actual) extreme hardiness. However, this evidences a perception "assignment problem" with tardigrades: as they branched off of the evolutionary tree extremely long ago, it is difficult to place them in a conventional context, e.g. the "arthropods" spiders, crabs, etc., to which they are likely distantly related, but do not belong.
They have been found in diverse regions of Earth's biosphere – mountaintops, the deep sea, tropical rainforests, and the Antarctic. Tardigrades are among the most resilient animals known, with individual species able to survive extreme conditions – such as exposure to extreme temperatures, extreme pressures (both high and low), air deprivation, radiation, dehydration, and starvation – that would quickly kill most other known forms of life. Tardigrades have survived exposure to outer space. There are about 1,300 known species in the phylum Tardigrada, a part of the superphylum Ecdysozoa consisting of animals that grow by ecdysis such as arthropods and nematodes. The earliest known true members of the group are known from Cretaceous (145 to 66 million years ago) amber, found in North America, but are essentially modern forms, and therefore likely have a significantly earlier origin, as they diverged from their closest relatives in the Cambrian, over 500 million years ago.
Tardigrades are usually about 0.5 mm (0.020 in) long when fully grown. They are short and plump, with four pairs of legs, each ending in claws (usually four to eight) or suction disks. Tardigrades are prevalent in mosses and lichens and feed on plant cells, algae, and small invertebrates. When collected, they may be viewed under a low-power microscope, making them accessible to students and amateur scientists.
