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Benefits ( Inglês )

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The history of safe use for Acetobacter aceti is predominately for food grade acetic acid (vinegar) production. Members of the genus Acetobacter have been used industrially since the 1850's (Edberg, 1991). A. aceti has also been reported in the literature as being used for cellulose production for specialty papers or headphones (Anonymous, 1989a, 1989b); however, strains capable of cellulose production are classified as A. pasteurianus or A. hansenii under the new taxonomic system (De Ley et al., 1984). A. aceti is considered a Class 1 Agent under the NIH Guidelines for Research Involving Recombinant DNA Molecules (U.S. Department of Health and Human Services, 1986), and is on the FDA's GRAS (generally recognized as safe) list of microorganisms. There are a number of (U.S. Environmental Protection Agency Toxic Substances Control Act) applications for acetic acid. These include manufacturing of acetate rayon, plastics production, rubber production, and photographic chemicals.
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Comprehensive Description ( Inglês )

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Acetobacter aceti is a Gram negative bacterium which is motile by peritrichous flagella. It is obligately aerobic possessing only the ability for respiratory metabolism with no fermentative ability. A. aceti does not form endospores. This bacterium is ubiquitous in the environment, existing in soil, water, flowers, fruits, and on honey bees; in essence, wherever sugar fermentation is occurring. A. aceti produces acetic acid from ethanol in alcoholic niches in the environment. Acetate and lactate are oxidized to CO2 and H2O by the organism. The optimal temperature for growth is between 25 to 30C, and the Ph optimum between 5.4 to 6.3 (De Ley et al., 1984). A. aceti is a common contaminant in all industrial fermentation facilities and is responsible for generating turbidity, ropiness, discoloration, and off-flavors in beer (Kough, 1991).
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Brief Summary ( Inglês )

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Acetobacter aceti is a benign microorganism that is ubiquitous in the environment, existing in alcoholic ecological niches such as flowers, fruits, honey bees, as well as in water and soil. It has a long history of safe use in the fermentation industry for the production of acetic acid from alcohol.
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Risks ( Inglês )

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A. aceti has not been reported as a human pathogen. It is ubiquitous in the environment, and therefore, comes in contact with humans on a frequent basis. Its optimum growth temperature is below that of the human body and its optimum pH is below that normally found on the surface of human skin. Due to its close association with sugar breakdown, it is unlikely that this species would form part of the normal bacterial flora of humans (Edberg, 1992). Review articles on the normal flora of the human body did not reveal A. aceti (Edberg, 1992). There are no reports in the literature that A. aceti is capable of producing toxins active against humans or animals, nor are there reports of A. aceti causing infection in humans or animals (Edberg, 1992). It does not produce enzymes or other extracellular factors normally associated with virulence. There is no reason to suspect that A. aceti could acquire or transfer any virulence factors. This bacterium does possess plasmids which are responsible for the production of enzymes used in acetic acid production. These plasmids have been shown to be transferred to other members of the species in the laboratory under optimal conditions. However, there is no evidence of plasmid transfer between strains of A. aceti or related species in the environment. Its unique ecological niches are such that it is unlikely that a second recipient or donor microorganism would be present in quantities sufficient for plasmid exchange to occur (Edberg, 1992). Biochemical characteristics of A. aceti virtually preclude it as being a threat to human health. Although it grows well with ethanol as a source of carbon, glucose has been shown to actually decrease the growth rate in culture, especially when other carbon sources were present (O'Sullivan and Ettlinger, 1976). In addition, industrial strains may have been selected so that they do not have the ability to grow on glucose (Weber and Ettlinger, 1971) or so that they utilize very specific amino acids as nitrogen sources. This may result in growth inhibition in the presence of alternate amino acids (O'Sullivan, 1974). In summary, A. aceti has no demonstrated virulence factors. It is not part of the normal flora of human skin or the body and is not expected to survive in a human host for sustained periods of time. The only threat to human health would lie in a massive contamination event in which workers may be exposed to extraordinarily high concentrations of the bacterium, andperhaps, develop an allergic or immunological reaction. It appears, however, because the bacterium is used for acetic acid production, should such a contamination event occur, the acetic acid would present a greater threat to workers than the bacterium itself. The potential for human virulence is virtually nonexistent for A. aceti (Edberg, 1992).
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Acetobacter aceti ( Inglês )

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Acetobacter aceti is a Gram-negative bacterium that moves using its peritrichous flagella. Louis Pasteur proved it to be the cause of conversion of ethanol to acetic acid in 1864. It is a benign microorganism which is present everywhere in the environment, existing in alcoholic ecological niches which include flowers, fruits, and honey bees, as well as in water and soil. It lives wherever sugar fermentation occurs.[1] It grows best in temperatures that range from 25 to 30 degrees Celsius and in pH that ranges from 5.4 to 6.3. For a long time it has been used in the fermentation industry to produce acetic acid from alcohol. Acetobacter aceti is an obligate aerobe, which means that it requires oxygen to grow.[2]

Acetobacter aceti is economically important because it is used in the production of vinegar by converting the ethanol in wine or cider into acetic acid. The acetic acid created by A. aceti is also used in the manufacturing of acetate rayon, plastics production, rubber production, and photographic chemicals. A. aceti is considered an acidophile, which means it is able to survive in acidic environments, due to having an acidified cytoplasm which makes nearly all proteins in the genome to evolve acid stability. A. aceti has become important in helping to understand the process by which proteins can attain acid stability.[2]

Industrial use

Acetic acid production

A. aceti is used for the mass production of acetic acid, the main component in vinegar. During the fermentation process of vinegar production, it is used to act on wines and ciders resulting in vinegar with acetic acid. It can be converted by a silicone tube reactor, which aids the fermentation process with oxidation.[3]

Safety

A. aceti has not been reported as a human pathogen. Human skin does not provide the bacteria with the optimal conditions for it to grow, which makes it safe to handle in factories that use the species to produce acetic acid. However, some evidence indicates it can be harmful to plants and other flora, though it exists naturally in the world.[1]

Growth

Oxidation is used to stimulate the growth of the A. aceti. Samples of the bacteria are placed in a few silicone tubes. These tubes are permeable to oxygen, after which they are left in a region warmer than the typical room temperature and cultured.[3]

Pink disease in pineapples

Because A. aceti occurs naturally and is widespread in the world, so far, no evidence shows it is a threat to humans, but in recent studies, it has been suspected to cause some detrimental effects on pineapples. The pink disease in pineapples causes the fruit to turn a slight pink color, only to eventually become brown and then rot. Similar experiments have also been tested on other fruits such as apples and pears and results end with rotten fruits. However, the bacterium seems to only be effective if the fruit has any locations exposing its flesh and the temperature surrounding its invasion is warmer than average. With the discovery of other Acetobacter species, skepticism exists regarding A. aceti being the only cause of the pink discoloration disease in pineapples. Studies are still being conducted on other species on the genus Acetobacter because 15 other species have been found in rotting fruits, as well.[1]

References

  1. ^ a b c "Acetobacter aceti Final Risk Assessment – Biotechnology Program Under Toxic Substances Control Act (TSCA) – US EPA". epa.gov. 27 September 2012. Retrieved 1 June 2013.
  2. ^ a b "Vinegar Production - FOOD ENGINEERING". 20 May 2013. Retrieved 1 June 2013.
  3. ^ a b Toda, Kiyoshi; Asakura, Tomoko (June 1994). "Acetic acid production by Acetobacter aceti in a silicone tube bioreactor". Biotechnology Letters. 16 (6): 617–620. doi:10.1007/BF00128610. S2CID 30117732.

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Acetobacter aceti: Brief Summary ( Inglês )

fornecido por wikipedia EN

Acetobacter aceti is a Gram-negative bacterium that moves using its peritrichous flagella. Louis Pasteur proved it to be the cause of conversion of ethanol to acetic acid in 1864. It is a benign microorganism which is present everywhere in the environment, existing in alcoholic ecological niches which include flowers, fruits, and honey bees, as well as in water and soil. It lives wherever sugar fermentation occurs. It grows best in temperatures that range from 25 to 30 degrees Celsius and in pH that ranges from 5.4 to 6.3. For a long time it has been used in the fermentation industry to produce acetic acid from alcohol. Acetobacter aceti is an obligate aerobe, which means that it requires oxygen to grow.

Acetobacter aceti is economically important because it is used in the production of vinegar by converting the ethanol in wine or cider into acetic acid. The acetic acid created by A. aceti is also used in the manufacturing of acetate rayon, plastics production, rubber production, and photographic chemicals. A. aceti is considered an acidophile, which means it is able to survive in acidic environments, due to having an acidified cytoplasm which makes nearly all proteins in the genome to evolve acid stability. A. aceti has become important in helping to understand the process by which proteins can attain acid stability.

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Acetobacter aceti ( Italiano )

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L'Acetobacter aceti è un microrganismo acidofilo resistente perfino a pH 0.

È un batterio aerobio obbligato, Gram - negativo, mobile grazie alla presenza di flagelli peritrici in grado di tollerare una concentrazione di etanolo del 10% (è pertanto il maggior responsabile delle ossidazioni dei vini a basso grado alcolico). La temperatura ottimale è compresa tra 25 e 30 °C, il pH ideale tra 5,4 e 6,3. In natura occupa le nicchie ecologiche nelle quali si sviluppa alcol in seguito a fermentazione degli zuccheri, come fiori, frutti, favi di api, ma può essere rinvenuto anche nel suolo o nelle acque. Attualmente sono descritte 4 sottospecie di Acetobacter aceti:[3]

  • Acetobacter aceti subsp. aceti
  • Acetobacter aceti subsp. liquefaciens
  • Acetobacter aceti subsp. orleanensis
  • Acetobacter aceti subsp. xylinum

Impieghi industriali

Viene impiegato industrialmente per la produzione di aceto, sfruttando la sua capacità di convertire l'etanolo in acido acetico. Non possiede invece abilità fermentative. È un contaminante molto comune in tutti i processi di fermentazione industriale, ed è responsabile di decolorazione, intorbidamento e sviluppo di odori sgradevoli nelle birre.

Rischi tossicologici e ambientali

Pericolosità per l'uomo e gli animali

Acetobacter aceti non è considerato patogeno per l'uomo. È ubiquitario nell'ambiente e può quindi naturalmente entrare in contatto con il corpo umano, ma la sua temperatura ottimale e il suo pH ottimale sono inferiori a quelli riscontrati normalmente sugli esseri umani. Grazie al fatto che necessita di zuccheri in fermentazione per sopravvivere non è associato alla normale microflora umana. Non vi sono casi conosciuti di patologie provocate da Acetobacter aceti, né di produzione di tossine pericolose per l'uomo o per gli animali.

Pericolosità per le piante

È stato segnalato (insieme a Erwinia herbicola, Gluconobacter oxydans e altri batteri) come responsabile del pink disease su ananas.[4][5] La malattia è caratterizzata da decolorazione del frutto che tende successivamente a diventare brunastro in seguito a lavorazione e conservazione.

È inoltre coinvolto in marciumi di mele e pere, nelle quali causa imbrunimenti di varia intensità.[6]

Note

  1. ^ (FR) L. Pasteur, Mémoire sur la fermentation acétique (PDF), in Annales scientifiques de l'Ecole normale superieure. Paris, Ser.1, vol. 1, 1864, pp. 113-158. URL consultato il 17 ottobre 2009.
  2. ^ (DE) M Beijerinck, Über die Arten der Essigbakterien, in Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Abteilung II, vol. 4, 1898, pp. 209-216.
  3. ^ Approved Lists of Bacterial Names, su ncbi.nlm.nih.gov.
  4. ^ H.L. Lyon, A survey of the pineapple problems, in Hawaiian Palnt Records, vol. 13, 1915, pp. 125-139.
  5. ^ (EN) K.G. Rohrbach, Pfeiffer, J.B., The interaction of four bacteria causing pink disease of pineapple with several pineapple (PDF), in Phytopathology, vol. 66, n. 4, aprile 1976, pp. 396-399, DOI:10.1094/Phyto-66-396. URL consultato il 17 ottobre 2009 (archiviato dall'url originale il 15 maggio 2009).
  6. ^ (EN) C. Van Keer, et al., Acetic Acid Bacteria as Causal Agents of Browning and Rot of Apples and Pears. (abstract), in Zentralbl. Bakteriol., Mikrobiol. Hyg., I Abt. C., vol. 2, n. 2, 1981, pp. 197-204. URL consultato il 22 ottobre 2020 (archiviato dall'url originale il 22 luglio 2012).

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Acetobacter aceti: Brief Summary ( Italiano )

fornecido por wikipedia IT

L'Acetobacter aceti è un microrganismo acidofilo resistente perfino a pH 0.

È un batterio aerobio obbligato, Gram - negativo, mobile grazie alla presenza di flagelli peritrici in grado di tollerare una concentrazione di etanolo del 10% (è pertanto il maggior responsabile delle ossidazioni dei vini a basso grado alcolico). La temperatura ottimale è compresa tra 25 e 30 °C, il pH ideale tra 5,4 e 6,3. In natura occupa le nicchie ecologiche nelle quali si sviluppa alcol in seguito a fermentazione degli zuccheri, come fiori, frutti, favi di api, ma può essere rinvenuto anche nel suolo o nelle acque. Attualmente sono descritte 4 sottospecie di Acetobacter aceti:

Acetobacter aceti subsp. aceti Acetobacter aceti subsp. liquefaciens Acetobacter aceti subsp. orleanensis Acetobacter aceti subsp. xylinum
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Acetobacter aceti ( Neerlandês; Flamengo )

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Acetobacter aceti is een soort van azijnzuurbacteriën behorende tot het geslacht Acetobacter.

Acetobacter aceti is de typesoort van het geslacht. De bacterie is gramnegatief en beweegt zich voort door middel van een flagellum. Louis Pasteur bewees in 1864 dat deze bacterie de oorzaak was van de omzetting van ethanol naar azijnzuur.[1] Het is een goedaardig micro-organisme dat alom aanwezig is in het milieu, in alcoholbevattende ecologische niches, bloemen, vruchten, bijen, alsook in water en bodems. De bacterie leeft waar fermentatie van suikers optreedt en groeit het best bij temperaturen tussen 25 en 30°C en een pH-waarde die varieert tussen 5,4 en 6,3. De bacterie wordt al sinds lange tijd gebruikt in de fermentatie-industrie om azijnzuur te produceren uit alcohol. Acetobacter aceti is obligaat aëroob, wat betekent dat hij zuurstof nodig heeft om te groeien.

Ondersoorten

  • Acetobacter aceti aceti (Pasteur 1864) De Ley and Frateur 1974
  • Acetobacter aceti liquefaciens (Asai 1935) De Ley and Frateur 1974
  • Acetobacter aceti orleanensis (Henneberg 1906) De Ley and Frateur 1974
  • Acetobacter aceti xylinus (Brown 1886) De Ley and Frateur 1974

Externe links

Bronnen, noten en/of referenties
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Acetobacter aceti: Brief Summary ( Neerlandês; Flamengo )

fornecido por wikipedia NL

Acetobacter aceti is een soort van azijnzuurbacteriën behorende tot het geslacht Acetobacter.

Acetobacter aceti is de typesoort van het geslacht. De bacterie is gramnegatief en beweegt zich voort door middel van een flagellum. Louis Pasteur bewees in 1864 dat deze bacterie de oorzaak was van de omzetting van ethanol naar azijnzuur. Het is een goedaardig micro-organisme dat alom aanwezig is in het milieu, in alcoholbevattende ecologische niches, bloemen, vruchten, bijen, alsook in water en bodems. De bacterie leeft waar fermentatie van suikers optreedt en groeit het best bij temperaturen tussen 25 en 30°C en een pH-waarde die varieert tussen 5,4 en 6,3. De bacterie wordt al sinds lange tijd gebruikt in de fermentatie-industrie om azijnzuur te produceren uit alcohol. Acetobacter aceti is obligaat aëroob, wat betekent dat hij zuurstof nodig heeft om te groeien.

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