Isoëtes tuckermanii may be an allotetraploid like
I. riparia, but it appears to have had different parental species. Variation may also have resulted from multiple allotetraploid origins. Typical plants of
I. tuckermanii have thin, soft leaves. More northern plants, referred to as
I. tuckermanii var.
borealis A. A. Eaton, have thick, stiff leaves.
Isoëtes tuckermanii hybridizes with I. echinospora; I. engelmannii [ = I. x foveolata A. A. Eaton ex Dodge]; and I. lacustris [ = I. x harveyi A. A. Eaton].