Spring Snails

Figures 7-10. Daphniola eptalophos sp. n. shell images from SEM Shell habitus 7, 8 protoconch 9 teleoconch 10 Scale bar 0.5 mm in Figs 7, 9 and 0.05 mm in Figs 8,10.

Figure 1.Bayesian tree based on the combined (COI, NDI) dataset. Nodes having posterior probabilities >95% are identified by filled circles. Specimen codes are from Appendix I.

Figure 3.Hydrobioids collectedduring the survey in mainland and insular Greece. A Daphniola exigua (dorsal and ventral view) B Isimerope semele (dorsal and ventral view, Megali Vrysi) C Pseudoislamia balcanica (dorsal and ventral view, Ag. Sophia) D Pseudamnicola pieperi (Olympos) E Radomaniola cf. curta (spring of Louros river) F Radomaniola cf. curta (Ag. Sophia spring) G Trichonia trichonica H Pseudobithynia eubooensis. Scale bar 1 mm.

Figure 4.Penes (dorsal, ventral surfaces). A, B Pyrgulopsis marilynae n. sp., USNM 883175 C, D Pyrgulopsis similis n. sp., USNM 1135065 E, F Pyrgulopsis gilae, BellMNH 20898. Scale bars A–F 200 µm. Dg2 dorsal gland along left edge Dg3 dorsal gland along right distal edge Ipg inner (left) penial gland Opg outer (right) penial gland Tg terminal gland Vg ventral gland.

Figure 4.Penes (dorsal, ventral surfaces). A, B Pyrgulopsis licina sp. n., USNM 850346 C, D Pyrgulopsis perforata sp. n., BellMNH 20891 E, F Pyrgulopsis sanchezi sp. n., USNM 883361 G, H Pyrgulopsis micrococcus, BellMNH 20663 I, J Pyrgulopsis turbatrix, USNM 860699 K, L Pyrgulopsis turbatrix, USNM 883373. Scale bars A–C 250 µm; D–L 500 µm. Pd penial duct Pf penial filament Pl penial lobe Tg terminal gland.

Figure 4.Graecoanatolica vegorriticola A Krya spring, Voiotia B Pigi Chariton, Voiotia C Lake Vegorritis, Pella. Scale bar 1 mm.

Figure 5.Shells, opercula and radula, Pyrgulopsis similis n. sp. A Holotype, USNM 1135064 B Shell, USNM 854684 C, D Opercula (outer, inner sides), USNM 1135065 E Portion of radular ribbon, USNM 1135065 F Central teeth, USNM 1135065 G Lateral and inner marginal teeth, USNM 1135065. Scale bars A, B 1.0 mm; C, D 200 µm; E 20 µm, F, G 10 µm.

Figure 6.Shells, opercula and radula, Pyrgulopsis perforata sp. n. A Holotype, USNM 853507 B, C Shells, USNM 1204734 D, E Opercula (outer, inner sides), USNM 857965 F Portion of radular ribbon, USNM 857965 G Central teeth, USNM 857965 H Lateral and inner marginal teeth, USNM 857965. Scale bars A–C 1.0 mm; D, E 200 µm; F 20 µm; G, H 10 µm.

Figure 3.Hydrobioids collectedduring the survey in mainland and insular Greece. A Daphniola exigua (dorsal and ventral view) B Isimerope semele (dorsal and ventral view, Megali Vrysi) C Pseudoislamia balcanica (dorsal and ventral view, Ag. Sophia) D Pseudamnicola pieperi (Olympos) E Radomaniola cf. curta (spring of Louros river) F Radomaniola cf. curta (Ag. Sophia spring) G Trichonia trichonica H Pseudobithynia eubooensis. Scale bar 1 mm.

Figure 1.Map showing the distribution of mtDNA clades I-III.

Figure 2.Map showing the distribution of mtDNA clades A–F with color codes matching those in Fig. 1.

Figure 5.A Pseudamnicola negropontina B Pseudamnicola macrostoma. The first whorls of the shells of Pseudamnicola negropontina wereheavily encrusted with epibionts. Scale bar 1 mm.

Figure 2.Bayesian tree based on the combined (COI, NDI) dataset. Posterior probabilities for nodes are given when >95%. Specimen codes are from the Table 1.

Figure 5.Photographs of habitats. A Spring south of Clay Pits, Ash Meadows, Nye County, Nevada, type locality of Pyrgulopsis licina sp. n. (photograph taken on 7/VII/1986) B Purgatory Spring, Ash Meadows, Nye County, Nevada, type locality of Pyrgulopsis sanchezi (15/XI/2011) C, D Uppermost spring east of Scotty’s Castle, Death Valley, Inyo County, California, type locality of Pyrgulopsis perforata sp. n. (18/IV/1980).

Figure 3.Hydrobioids collectedduring the survey in mainland and insular Greece. A Daphniola exigua (dorsal and ventral view) B Isimerope semele (dorsal and ventral view, Megali Vrysi) C Pseudoislamia balcanica (dorsal and ventral view, Ag. Sophia) D Pseudamnicola pieperi (Olympos) E Radomaniola cf. curta (spring of Louros river) F Radomaniola cf. curta (Ag. Sophia spring) G Trichonia trichonica H Pseudobithynia eubooensis. Scale bar 1 mm.

Figure 4.Penes (dorsal, ventral surfaces). A, B Pyrgulopsis marilynae n. sp., USNM 883175 C, D Pyrgulopsis similis n. sp., USNM 1135065 E, F Pyrgulopsis gilae, BellMNH 20898. Scale bars A–F 200 µm. Dg2 dorsal gland along left edge Dg3 dorsal gland along right distal edge Ipg inner (left) penial gland Opg outer (right) penial gland Tg terminal gland Vg ventral gland.

Figure 1.Bayesian tree based on the combined (COI, NDI) dataset. Nodes having posterior probabilities >95% are identified by filled circles. Specimen codes are from Appendix I.

Figure 1.Map showing the distribution of mtDNA clades I-III.

Figure 4.Penes (dorsal, ventral surfaces). A, B Pyrgulopsis licina sp. n., USNM 850346 C, D Pyrgulopsis perforata sp. n., BellMNH 20891 E, F Pyrgulopsis sanchezi sp. n., USNM 883361 G, H Pyrgulopsis micrococcus, BellMNH 20663 I, J Pyrgulopsis turbatrix, USNM 860699 K, L Pyrgulopsis turbatrix, USNM 883373. Scale bars A–C 250 µm; D–L 500 µm. Pd penial duct Pf penial filament Pl penial lobe Tg terminal gland.

Figure 6.Shells, Pyrgulopsis gilae. A USNM 1135052 B USNM 854574. Scale bars A, B, 1.0 mm.

Figure 7.Shells, opercula and radula, Pyrgulopsis sanchezi sp. n. A Holotype, USNM 850333 B–D Shells, USNM 853505, USNM 1204755, USNM 853501 E, F Opercula (outer, inner sides), USNM 883361 G Portion of radular ribbon, USNM 883361 H Central teeth, USNM 883361 I Lateral and inner marginal teeth, USNM 883361. Scale bars A–D 1.0 mm; E, F 100 µm; G 20 µm; H, I 10 µm.

Figure 2.Map showing the distribution of mtDNA clades A–F with color codes matching those in Fig. 1.

Figure 5.Photographs of habitats. A Spring south of Clay Pits, Ash Meadows, Nye County, Nevada, type locality of Pyrgulopsis licina sp. n. (photograph taken on 7/VII/1986) B Purgatory Spring, Ash Meadows, Nye County, Nevada, type locality of Pyrgulopsis sanchezi (15/XI/2011) C, D Uppermost spring east of Scotty’s Castle, Death Valley, Inyo County, California, type locality of Pyrgulopsis perforata sp. n. (18/IV/1980).

Figure 1.Bayesian tree based on the combined (COI, NDI) dataset. Nodes having posterior probabilities >95% are identified by filled circles. Specimen codes are from Appendix I.