Plaque assay dilution series

Description:

Description: Photograph of viral plaque formation to count viral titer (plaque assay). Vero cells, which grew confluently on the bottom of the 24-well plastic plate (1.5 cm diameter each), were infected with serially diluted solutions containing herpes simplex virus, and then cultured over night to make viral plaque. The number of plaques indicates the number of the infectious virus (= viral titer, as plaque forming unit). Photo: 4-fold dilution series from top-left to bottom-right. Living cells were stained with crystal violet. The viral plaques, each was from one virion, remained transparent. Date: 1 March 2006. Source: Own work. Author: Y tambe. Permission(Reusing this file): GFDL, CC-by-sa 2.5,2.0,1.0. Other versions: Image:CPE_rounding.jpg; Microscopic image. Image:Plaque_assay_macro.jpg; Macroscopic photograph of a well.