Pone.0082774.g003

Description:

Summary.mw-parser-output table.commons-file-information-table,.mw-parser-output.fileinfotpl-type-information{border:1px solid #a2a9b1;background-color:#f8f9fa;padding:5px;font-size:95%;border-spacing:2px;box-sizing:border-box;margin:0;width:100%}.mw-parser-output table.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{vertical-align:top}.mw-parser-output table.commons-file-information-table>tbody>tr>td,.mw-parser-output table.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:4px}.mw-parser-output.fileinfo-paramfield{background:#ccf;text-align:right;padding-right:0.4em;width:15%;font-weight:bold}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{border-top:0;padding-top:0;margin-top:-8px}@media only screen and (max-width:719px){.mw-parser-output table.commons-file-information-table,.mw-parser-output.commons-file-information-table.fileinfotpl-type-information{border-spacing:0;padding:0;word-break:break-word;width:100%!important}.mw-parser-output.commons-file-information-table>tbody,.mw-parser-output.fileinfotpl-type-information>tbody{display:block}.mw-parser-output.commons-file-information-table>tbody>tr>td,.mw-parser-output.commons-file-information-table>tbody>tr>th,.mw-parser-output.fileinfotpl-type-information>tbody>tr>td,.mw-parser-output.fileinfotpl-type-information>tbody>tr>th{padding:0.2em 0.4em;text-align:left;text-align:start}.mw-parser-output.commons-file-information-table>tbody>tr,.mw-parser-output.fileinfotpl-type-information>tbody>tr{display:flex;flex-direction:column}.mw-parser-output.commons-file-information-table+table.commons-file-information-table,.mw-parser-output.commons-file-information-table+div.commons-file-information-table>table{margin-top:-1px}.mw-parser-output.fileinfo-paramfield{box-sizing:border-box;flex:1 0 100%;width:100%}} Description: English: Dinoflagellate/Viral NucleoProtein (DVNP) immunofluorescence assays (IFAs) of ethanol-fixed haemolymph samples obtained from heavily infected blue swimmer crabs Callinectes sapidus from Chesapeake Bay (USA).DNA is shown in red and anti-DVNP is shown in green.(A1) IFA (left) and brightfield overlay (right) of a DVNP-negative C. sapidus hyalinocyte blood cell.(A2) IFA (left) and brightfield overlay (right) of a DVNP-negative C. sapidus granulocyte blood cell.(A3) IFA (left) and brightfield overlay (right) of a DVNP-positive Hematodinium cell.(B1) HematodiniumÄÄ cells are strongly labeled around the edge of their nuclei while the DNA stain concentrates in the center of the nuclei.(B2) DVNP-labeling is absent in C. sapidus cells (red nuclei) and prominent in Hematodinium cells (green-yellow nuclei). Date: 6 December 2013. Source: New Host Range for Hematodinium in Southern Australia and Novel Tools for Sensitive Detection of Parasitic Dinoflagellates. In: PLOS ONE 8(12): e82774. doi:10.1371/journal.pone.0082774. Author: Sebastian G. Gornik, Andrea Cranenburgh, Ross F. Waller.